WebApr 13, 2024 · Contrary to our prediction, CD11b expression was similar between BM neutrophils from neonatal mice housed in germ-free and specific pathogen-free conditions (Figure S1D). This suggests that the increase in CD11b expression on neonatal neutrophils was driven by developmentally intrinsic mechanisms that are independent of microbial … WebApr 11, 2024 · On the contrary, little ... This was followed by 3× PBS washes and incubation with fluorophore-conjugated secondary antibodies (diluted in blocking buffer) for another hour at RT in the dark. Slides were then washed again 3× with PBS and imaged on a Zeiss LSM 880 confocal microscope using a 63× oil immersion lens.

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To The Contrary PBS

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