Cytological profiling

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August undefined, 2024

WebDec 2, 2013 · Computational methods for image-based profiling are under active development, but their success hinges on assays that can capture a wide range of … WebAug 22, 2024 · Understanding the mechanism of action (MOA) of new antimicrobial agents is a critical step in drug discovery but is notoriously difficult for compounds that appear to inhibit multiple cellular pathways. We recently described image-based approaches [bacterial cytological profiling and rapid inducible profiling (RIP)] for identifying the cellular …

High-Resolution Bacterial Cytological Profiling Reveals …

WebDec 9, 2015 · We have used cytological profiling to determine the cellular target for four newly purified or partially characterized compounds, including the cannibalistic … WebApr 12, 2024 · Age-related hearing loss (ARHL) negatively impacts quality of life in the elderly population. The prevalent cause of ARHL is loss of mechanosensitive cochlear … eternity collar silver

Profiling the effect of nafcillin on HA-MRSA D712 using …

WebSep 17, 2013 · A powerful emerging technique based on cytological profiling has been successfully used to identify the cellular pathways targeted by the inhibitors (Nonejuie et al. 2013; Martin et al. 2024 ... WebApr 12, 2024 · Using RNA-seq, advanced imaging, and electrophysiology, Liu et al. reveal molecular and cytological profiles of aging cochlear hair cells. Their study also suggests … WebCytological profiling (CP) is an unbiased image-based screening technique that uses automated microscopy and image analysis to profile compounds based on numerous quantifiable phenotypic features. We used CP to evaluate a library of nearly 500 … eternity collars for subs

Multiplex cytological profiling assay to measure diverse cellular ...

Multiplex Cytological Profiling Assay to Measure Diverse ... - PLOS

WebSep 25, 2015 · The second, nocapyrone L (6), possessed a cytological profile that clustered with known calcium channel modulators, in line with previous published … WebCytological profiling (CP) is an unbiased image-based screening technique that uses automated microscopy and image analysis to profile compounds based on numerous quantifiable phenotypic features. We used CP to evaluate a library of nearly 500 compounds with documented mechanisms of action (MOAs) spanning a wide range of biological … eternity cologne 6.7 ozWebSep 18, 2024 · Screening ecosystems containing at least one antiviral sample cytological profile were cross-referenced to cytological profiles obtained by the same method from a library of 720 bioactive reference compounds (Bray et al. 2024; Pennisi 2016). Orthogonal analyses using cytological profiling revealed HIV-inhibitory MeE samples in four major … eternity collars women

"WebHigh-content screening (HCS) in cell-based systems uses living cells as tools in biological research to elucidate the workings of normal and diseased cells. HCS is also used to discover and optimize new drug candidates. High content screening is a combination of modern cell biology, with all its molecular tools, with automated high resolution … " - Cytological profiling

Cytological profiling

WebBacterial Cytological Profiling (BCP) as a Rapid and Accurate Antimicrobial Susceptibility Testing Method for Staphylococcus aureus Authors : D.T. Quach 1,4 , G. Sakoulas 2 , V. … Webthe multiplex cytological profiling protocol, images were captured at 20x magnification with an automated epifluorescent microscope. We extracted 824 morphological features (Table S2) from each cell using the open-source software CellProfiler [6]. A number of cellular phenotypes could be detected by eye (Figure 1B).

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WebDec 1, 2024 · The other platform, a cytological profiling (CP) technology developed by Lokey's lab, involves high-content image analysis of cells exposed to the samples being screened and then stained with a ... WebCytological profiling (CP) is an unbiased image-based screening technique that uses automated microscopy and image analysis to profile compounds based on numerous …

WebBacterialcytologicalpro lingrapidlyidenti esthecellularpathwaystargetedbyantibacterialmolecules. PoochitNonejuiea,MichaelBurkartb,KitPoglianoa,andJoePoglianoa,1 WebJan 21, 2024 · We recently developed Bacterial Cytological Profiling (BCP) as a new whole-cell screening technique that can be used to rapidly identify the mechanism of action (MOA) of antibiotics [11–16]. BCP can accurately identify the pathway inhibited by antibacterial compounds present in unfractionated crude organic extracts and can be …

WebJul 23, 2024 · MorphEUS, like all cytological profiling techniques, is data-driven and based on classification among a pool of other profiles. It requires multiple representative profiles from M. tb treated with compounds known to target the same broad cellular target. As the drug set expands, the accuracy and resolution of MorphEUS will improve. WebMay 1, 2016 · Bacterial cytological profiling is a powerful tool for directing antibiotic discovery efforts because it can be used to determine the specificity of an antibiotic's in vivo mechanism of action and demonstrates that BCP can identify multiple pathways that are inhibited by an individual molecule.

WebDec 17, 2024 · Bacterial cytological profiling. At the 3 hour mark, samples were taken for fluorescence microscopy, similar to previously described with modifications 9,10,11.In brief, 8 µL cells were added to ...

WebJul 17, 2024 · In other bacterial species such as Escherichia coli, Bacillus subtilis, and Acinetobacter baumannii, profiling of cytological changes in response to drug treatment has yielded a rapid and resource-sparing procedure to determine drug mechanism (7–9).This method, known as bacterial cytological profiling (BCP), is based on the … fireflies fortniteWebFeb 25, 2024 · 2 23 Importance: We demonstrate that bacterial cytological profiling is a powerful tool for directing antibiotic discov- ery efforts because it can be used to … fireflies going extinctWebDec 2, 2013 · Computational methods for image-based profiling are under active development, but their success hinges on assays that can capture a wide range of … fireflies games columbia scWebNational Center for Biotechnology Information fireflies group named after nocturnal birdsWebApr 11, 2024 · Besides conventional growth inhibition assays, an interesting emerging technique is cytological profiling, which allows ‘function-first’ mining of natural products with desired biological activities by predicting the mechanisms of action based on microscopy analysis of the cellular responses of target strains (Nonejuie et al. 2013; … fireflies gorillazWebBacterial cytological profiling (BCP) is a rapid and powerful approach to identify the cellular pathway affected by antibacterial molecules. As a one-step assay, BCP presents … eternity comic vineWebSep 30, 2016 · Bacterial cytological profiling, using quantitative microscopy, is a powerful approach for identifying the mechanism of action of antibacterial molecules affecting different cellular pathways. eternity college