Webof 1.5x105 cells/well in 12-well plates (cat. no. CLS3513; Sigma-Aldrich), and 24 h later, cells were lysed with CelLytic M Cell Lysis Reagent (Sigma-Aldrich) for whole-cell protein extraction. Genomic DNA was sonicated for 15with an sec output frequency of 20 kHz and 50% amplitude, using a 150VT sonicator (Biologics, Inc, Cary, NC, USA). WebAfter the 1-week treatment, the animals were anaesthetized with isofluorane and perfused with ice-cold PBS. Immediately after dissection, the aorta, heart, liver, and skeletal …
Can I substitute MDA lysis buffer with CelLytic MT Cell Lysis Reagent ...
WebCelLytic MT is an efficient reagent for the extraction of proteins. The lysis buffer consists of a dialyzable mild detergent, bicine, and 150 mM NaCl, resulting in minimal interference … Web3. Add M-PER Reagent to the cell pellet. Use at least 1 ml of M-PER Reagent for each 100 mg (~100 µl) of wet cell pellet. If a large amount of cells is used, first add 1/10 the final recommended volume of M-PER Reagent to the cell pellet. Pipette the mixture up and down to resuspend pellet. Add the rest of the M-PER Reagent to the cell suspension. stp intake valve cleaner
CelLytic(TM) MT Cell Lysis Reagent from Sigma-Aldrich
WebFeb 1, 2024 · Frozen kidney samples were homogenized in ice-cold buffer (CelLytic™ MT Cell Lysis Reagent, Sigma–Aldrich) including 0.5 mmol/L Na 3 VO 4, a protease inhibitor cocktail (Complete mini, Roche Molecular Biochemicals) and 1 mmol/l phenylmethylsulfonyl fluoride. The homogenate was centrifuged at 15,000 g for 15 min at 4 °C to obtain the ... WebCelLytic MT is an efficient reagent for the extraction of proteins. The lysis buffer consists of a dialyzable mild detergent, bicine, and 150 mM NaCl, resulting in minimal interference … WebCelLytic MT is an efficient reagent for the extraction of proteins. The lysis buffer consists of a dialyzable mild detergent, bicine, and 150 mM NaCl, resulting in minimal interference with protein interactions and biological activity. CelLytic MT is also used for extraction of cell-line proteins. A volume of 20 mL of CelLytic MT is sufficient ... stp in software